Anti-pan Cytokeratin antibody [AE1/AE3] ab27988 from Abcam

Product Specs
ItemAnti-pan Cytokeratin antibody [AE1/AE3]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab27988
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IsotypeIgG1
Quantity500 UL
ClonalityMonoclonal
HostMouse, Nude mouse
CloneAE1/AE3
Conjugate/TagUnconjugated
Gene NameKRT18
ReactivityHuman
Targetpan Cytokeratin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Poor Outcome in Postpartum Breast Cancer Patients Is Associated with Distinct Molecular and Immunologic FeaturesClinical Cancer ResearchSeptember 15, 2023Hanne Lefrère, Kat Moore, Giuseppe Floris, Joyce Sanders, Iris M. Seignette, Tycho Bismeijer, Dennis Peters, Annegien Broeks, Erik Hooijberg, Kristel Van Calsteren, et al.No. VWRKILM3825-C1, clone B-A38, 1/100 dilution, 1 hour at RT), and anti-PanCK (Abcam, Catalog No. Ab27988, clone AE1/AE3, 1/100 dilution, 2 hours at RT). mIF staining was performed on a Ventana Discovery Ultra

(2) MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast CancerNature communicationsSeptember 13, 2023Doha ZO, Wang X, Calistri NL, Eng J, Daniel CJ, Ternes L, Kim EN, Pelz C, Munks M, Betts C, et al.#ab183991,1:1000); Granzyme B (Abcam #ab4059, 1:200); Ly6G (eBioscience #551459, 1:200); PAN Keratin (Abcam #ab27988, 1:100). CK5 (abcam, EP1601Y, 1:100); S100A6 (CST, D9F9D, 1:100); CD11c (CST, D1V9Y, 1:100); CD103(Biolegend,

(3) Deciphering the Role of p60AmotL2 in Epithelial Extrusion and Cell DetachmentCellsAugust 28, 2023Weiyingqi Cui, Aravindh Subramani, Pedro Fonseca, Yumeng Zhang, Le Tong, Yuanyuan Zhang, Lars Egevad, Andreas Lundqvist, Lars HolmgrenCaspase-3 (product number 9662S, Cell Signaling, Danvers, MA, USA), and cytokeratin (product number ab27988, Abcam, Cambridge, UK). The following secondary antibodies were utilized: Alexa 568 anti-mouse (product

(4) Three-Dimensional Breast Cancer Model to Investigate CCL5/CCR1 Expression Mediated by Direct Contact between Breast Cancer Cells and Adipose-Derived Stromal Cells or AdipocytesCancersJuly 5, 2023Watzling M, Klaus L, Weidemeier T, Horder H, Ebert R, Blunk T, Bauer-Kreisel P.adipocyte-spheroids. To visualize tumor cells in co-culture spheroids, pan-cytokeratin AE1 /AE3 (1:100; ab27988, Abcam) co-staining was carried out. CCL5 receptors were stained using αCCR1 and αCCR5 antibodies (1:200;

(5) Genomic landscape and expression profile of consensus molecular subtype four of colorectal cancerFrontiers in ImmunologyJune 19, 2023Yujie Lu, Dingyi Gu, Chenyi Zhao, Ying Sun, Wenjing Li, Lulu He, Xiaoyan Wang, Zhongyang Kou, Jiang Su, Feng GuoAnti-ZEB1 antibody [EPR17375] (ab203829, dilution 1:150, Abcam, Shanghai, China), Anti-pan Cytokeratin antibody [AE1/AE3] (ab27988, dilution 1:100, Abcam, Shanghai, China) for 20 min, respectively. Sections were washed by PBS, followed

(6) GC‑MSC‑derived circ_0024107 promotes gastric cancer cell lymphatic metastasis via fatty acid oxidation metabolic reprogramming mediated by the miR‑5572/6855‑5p/CPT1A axisOncology ReportsMay 29, 2023Lin Wang, Chen Wu, Juan Xu, Zhen Gong, Xiaoli Cao, Jiaying Huang, Haibo Dong, Wei Zhu, Feng Huang, Chenglin Zhou, et al.and were then separately incubated with primary antibodies against pan-cytokeratin (AE1/AE3; cat. no. ab27988, Abcam, dilution: 1:200) and CPT1A (cat. no. ab128568, Abcam, dilution: 1:200) overnight at 4°C. After

(7) Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasisCancer cell internationalMay 9, 2023Huang J, Wang X, Wen J, Zhao X, Wu C, Wang L, Cao X, Dong H, Xu X, Huang F, et al.ABclonal, China), CD44 (A12410, ABclonal, China), CPT1A (#12252, CST, USA), pan-cytokeratin (AE1/AE3) (ab27988, Abcam, UK), IL-8 (A2541, ABclonal, China) and STC1 (20621-1-AP, Proteintech, China) using instant SABC-POD

(8) T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interactionEMBO reportsMarch 6, 2023Patricia Himmels, Thi Thu Thao Nguyen, Maresa Caunt Mitzner, Alfonso Arrazate, Stacey Yeung, Jeremy Burton, Robyn Clark, Klara Totpal, Raj Jesudason, Angela Yang, et al.Elution was performed with Cell Conditioning Solution 2 followed by the primary antibody Pan Cytokeratin (ab27988, Abcam, Cambridge, MA) at 2 μg/ml for 32 min at 37°C. A rabbit recombinant anti‐IgG1+IgG2a+IgG2 antibody

(9) Niche stiffness sustains cancer stemness via TAZ and NANOG phase separationNature communicationsJanuary 16, 2023Liu X, Ye Y, Zhu L, Xiao X, Zhou B, Gu Y, Si H, Liang H, Liu M, Li J, et al.for 15 min. Afterward, samples were incubated with mouse anti-human CK (1:200, clone: AE1/AE3, cat# ab27988, Abcam), goat anti-human ALDH1 (1:100, cat# AF5869, R&D Systems), rabbit anti-human TAZ (1:100, clone:

(10) Potential of VEGFR2 expression as a predictive marker of PD‑1 blockade in patients with advanced NSCLCOncology ReportsOctober 20, 2022Kyoichi Kaira, Hisao Imai, Tomonori Kawasaki, Kousuke Hashimoto, Yu Miura, Ayako Shiono, Ou Yamaguchi, Atsuto Mouri, Kunihiko Kobayashi, Masanori Yasuda, et al.236A/E7; pH 6 retrieval) (cat. Ab20034) and pan cytokeratin (Abcam; clone AE1/AE3; 1:100; pH 6) (cat. Ab27988). Blocking reagent (Antibody Diluent, Akoya) was incubated at room temperature, 10 min. Secondary antibody

(11) A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor ImmunityCancer discoveryOctober 5, 2022Abrego J, Sanford-Crane H, Oon C, Xiao X, Betts CB, Sun D, Nagarajan S, Diaz L, Sandborg H, Bhattacharyya S, et al.species,Rabbit,Rabbit,Rabbit,Rabbit AEC,23 min,17 min,10 min,24 min ,,,,Round 2 Target,,,,panCK Company/Product,,,,Abcam (ab27988) Clone,,,,AE1/1E3 Dilution,,,,1/100 Duration,,,,"RT, 1 hr" Secondary species,,,,Mouse AEC,,,,22

(12) Cytotoxic CD8(+) T cells may be drivers of tissue destruction in Sjögren's syndromeScientific reportsSeptember 14, 2022Kaneko N, Chen H, Perugino CA, Maehara T, Munemura R, Yokomizo S, Sameshima J, Diefenbach TJ, Premo KR, Chinju A, et al.Cell Signaling Technology), anti-GZMA (1:800) (clone: LS-C312742; LSBio), anti-pan-CK (1:200) (clone: ab27988; Abcam), anti-AQP5 (1:500) (clone: ab92320; Abcam), anti-HLA-DR (1:1000) (clone: ab20181; Abcam), anti-NKp46

(13) Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot StudyInternational Journal of Molecular SciencesSeptember 1, 2022Munir A. Al-Zeer, Franziska Prehn, Stefan Fiedler, Ulrich Lienert, Michael Krisch, Johanna Berg, Jens Kurreck, Guido Hildebrandt, Elisabeth SchültkeFor the cellular characterization of epithelial cells, pan-cytokeratin (anti-pan Cytokeratin, abcam, ab27988, 1:250) was used. This was followed by incubation with corresponding secondary antibodies (Alexa Fluor

(14) Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosisFrontiers in PharmacologyAugust 31, 2022Måns Kadefors, Frida Berlin, Marie Wildt, Göran Dellgren, Sara Rolandsson Enes, Anders Aspberg, Gunilla Westergren-Thorssonclone EPR3132, Abcam, ab92574) or against DPP4/CD26 and pan-cytokeratin (1:100, clone AE1/AE3, Abcam, ab27988) diluted in PBS with 2% BSA at 4 °C overnight or at 37 °C for 1 h in a humid chamber. Sections were

(15) Patient Derived Organoids Confirm That PI3K/AKT Signalling Is an Escape Pathway for Radioresistance and a Target for Therapy in Rectal CancerFrontiers in OncologyJuly 4, 2022Kasun Wanigasooriya et al.PDOs and their respective tumour of origin were stained with antibodies targeting pan-Cytokeratin (ab27988, Abcam, UK) and CDX2 (9272S, Abcam, UK) following standard protocols. The primary antibody dilution

(16) Plasma CXCL14 as a Candidate Biomarker for the Diagnosis of Lung CancerFrontiers in OncologyJune 8, 2022Peng-Fei Tian et al.were incubated with anti-CXCL14 antibody and Anti-pan Cytokeratin antibody (1:200, Lot ab137541 and ab27988; Abcam, Cambridge, UK) overnight at 4°C. After incubation with secondary antibody (Lot 715-585-150 and

(17) Temporal changes in T cell subsets and expansion of cytotoxic CD4+ T cells in the lungs in severe COVID-19Clinical ImmunologyApril 1, 2022Naoki Kaneko et al.with the following antibodies: anti-CD3 (A045229–2; DAKO), anti-CD4 (ab133616; Abcam), anti-Pan-CK (ab27988; Abcam), anti-CD31 (3528; Cell Signaling Technology), anti-SLAMF7 (HPA055945; Sigma-Aldrich), anti-CX3CR1

(18) Significance of cancer stroma for bone destruction in oral squamous cell carcinoma using different cancer stroma subtypesOncology reportsApril 1, 2022Shan Q, Takabatake K, Kawai H, Oo MW, Inada Y, Sukegawa S, Fushimi S, Nakano K, Nagatsuka H.temperature and incubated with primary antibodies, including mouse anti-pan-cytokeratin (AE1/3; cat. no. ab27988; 1:20; Abcam), anti-matrix metalloproteinase (MMP)9 (F-69; 1:20; Kyowa Pharma Chemical Co., Ltd.) anti-membrane-type

(19) Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19iScienceMarch 18, 2022Jun Wang et al.human CD3,Abcam,"Cat#ab17143, Clone F7.2.38; RRID:AB_782094" Mouse anti human cytokeratin,Abcam,"Cat#ab27988, Clone AE1/AE3; RRID:AB_10717335" ,, Biological samples,Biological samples,Biological samples ,, Covid

(20) Lymphocytes Infiltration and Expression of PD-1 and PD-L1 in Colorectal Cancer Between HIV-Infected and Non-HIV-Infected Patients: A Propensity Score Matched Cohort StudyFrontiers in OncologyMarch 2, 2022Ye Cao, Qian Wu, Shixian Lian, Li Deng(1:400, ab133616, Abcam), CD8 (1:200, ab21344, Abcam), CD56 (1:200, ab75813, Abcam), Pan-CK (1:500, ab27988, Abcam), PD-1 (1:200, ab52587, Abcam), and PD-L1 (1:100, ab213524, Abcam), with incubation time for

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Anti-pan Cytokeratin antibody [AE1/AE3] from Abcam

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Anti-pan Cytokeratin antibody [AE1/AE3] from Abcam

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